Thursday, March 26, 2015

Animal fun






Book:

Medical Mycology: Current Trends and Future Prospects

Mehdi Razzaghi-Abyaneh, Masoomeh Shams-Ghahfarokhi, Mahendra Rai

August 30, 2015 Forthcoming by CRC Press 
Reference - 400 Pages - 20 Color 
ISBN 9781498714211 - CAT# K25282

Features

  • Deals with current topics like mold allergy, fungal bio-films, and clinical aspects of fungi like Curvularia and Bipolaris
  • Incorporates global contribution by top authorities of the world
  • Includes current methods of identification of commercially important fungi, such as PCR and Mald-Tof
  • Discusses Paracoccidioidomycosis, Onychomycosis, Sporotrichosis, and other emerging infections by fungi
  • Covers past present and future of medical mycology with most topics

Summary

The identification of medically important fungi has been an important area of study that warrants further extensive research. The use oftraditional and molecular methods of identification, provides new insights into differentiation of species and ultimately the line of treatment can be determined. This book incorporates a diverse group of medically important fungi and diseases, including common dermatophytes, onychomycosis, Coccidiomycosis, Paracoccidioidomycosis, Mycotic keratitis, Sporotrichosis, Histoplasmosis and determination of identity of medically important fingi by uising modern techniques such as PCH and the use of MALDI-TOF: as a rapid and new approach in fungal diagnosis and differentiation.

Table of Contents

Section I: Superficial mycoses caused by molds and yeasts
Dermatophyte infections in human being:  The current trends and future prospectsMateja Dolenc-Voljč
Onychomycosis: Diagnosis and therapy; Shari R. Lipner and Richard K. Scher
Mycotic Keratitis: Current perspectives; Sabyasachi Bandyopadhyay and Mita Saha (Dutta Chowdhury)
Section II: Emerging mycoses caused by opportunistic fungal pathogens
Incidence of Candida species in urinary tract infections and their control by using bioactive compounds occurring in medicinal plants; Vaibhav Tiwari, Mamie Hui, and Mahendra Rai
Fungi in otorhinolaryngology-related fungal diseases: A convenient classification for better clinical practice; Ahmad Ragab
Fungal infection after kidney transplantation; Salwa, S., Abdul Razack Amir, and Samir Amr
Clinical importance of the genus CurvulariaKrizsán K Krisztina Krizsán, Tamás Papp, Palanisamy Manikandan, Subramanian Shobana, Csaba Vágvölgyi, and László Kredics
Section III: Classic mycoses caused by dimorphic fungi
Classic histoplasmosis; Recardo Negroni
Sporotrichosis: The state-of-the-art; Alexandro BonifazRubí Rojas-Padilla, Andrés Tirado-Sánchez, and Rosa M. Ponce
Paracoccidioidomycosis: An endemic mycosis in the Americas;Carlos Pelleschi Taborda, Martha Eugenia Uran J, and Luiz R. Travassos
Increased cases of valley fever disease in Central California: An update; Tara Dubey
Section IV: Fungal pathogenesis in biofilm and allergy
Fungal biofilms: Formation, resistance and pathogenicity;Janaina de Cássia Orlandi Sardi, Nayla de Souza Pitangui, Fernanda Patrícia Gullo, Ana Marisa Fusco-Almeida, and Maria Jose Soares Mendes-Giannini
Fungal allergens: Recent trends and future prospects; Idoia Postigo and Jorge Martínez
Section V: Novel diagnostic methods, susceptibility testing and miscellaneous mycoses
MALDI-TOF: A rapid and new approach in fungal diagnosis and susceptibility testing; Mehmet Ali SARACLI
Medical mycology in Iran: Past, present, and future;Mohammadhassan Gholami-Shabani, Masoomeh Shams-Ghahfarokhi, Mohammadreza Shidfar, and Mehdi Razzaghi-Abyaneh
Culture collection DPUA: Decades supporting diagnostic of fungal diseases in Amazonas, Brazil; Simas Teixeira, Kátia Santana Cruz, Iara Maria Bomfim, Renata de Almeida Lemos, Mircella Marialva Alecrim, Raimundo Felipe da Cruz Filho, Nélly Mara Vinhote Marinho, and Taciana de Amorim Silva
Evaluation of the Antibacterial Properties of Silver Nanoparticles synthesized with Fusarium Oxysporum and Escherichia coli

Author(s)Mohammad Hassan Gholami-ShabaniAfshin ImaniMohammad chamaniMahdi Razzaghi-AbyanehGholam Hossein RiaziMohsen Chian ,Azim Akbarzadeh

M.shabani@att.net

Aim and Background. Silver nanoparticles have antibacterial properties and are used in textile, hygiene, refinement, staining, agriculture and livestock industries. The aim of this study was the production of silver nanoparticles using Fusarium oxysporum and Escherichia coli.

Materials and Methods. We used agar diffusion disk for the inspection of antibacterialproperties of nanosilver colloids. The shape and morphology of nanosilver colloids were evaluated by transmission electron microscopy.


Results. The presence of nanosilver colloids was confirmed by UV/Vis spectrophotometry. The mean size of nanoparticles produced by F. oxysporum was smaller than those of them in E. coli.


Conclusion. Results of the present study showed that the quality of nanosilver colloids from F. oxysporum is better than those of them in E. coli.

KeywordsSilver nanoparticlesAntibacterial activityEscherichia coliFusarium oxysporum,

Cite: Gholami-Shabani M, Imani A, chamani M, Razzaghi-Abyaneh M, Riazi G, Chian M et al . Evaluation of the Antibacterial Properties of Silver Nanoparticles synthesized with Fusarium Oxysporum and Escherichia coli. NCMBJ. 2012; 2 (6) :27-33
Applied Biochemistry and Biotechnology
April 2014, Volume 172, Issue 8, pp 4084-4098

Antimicrobial Activity and Physical Characterization of Silver Nanoparticles Green Synthesized Using Nitrate Reductase from Fusarium oxysporum

Mohammadhassan Gholami-Shabani, Azim Akbarzadeh, Dariush Norouzian, Abdolhossein Amini, Zeynab Gholami-Shabani, Afshin Imani, Mohsen Chiani, Gholamhossein Riazi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh


Abstract

Nanostructures from natural sources have received major attention due to wide array of biological activities and less toxicity for humans, animals, and the environment. In the present study, silver nanoparticles were successfully synthesized using a fungal nitrate reductase, and their biological activity was assessed against human pathogenic fungi and bacteria. The enzyme was isolated from Fusarium oxysporum IRAN 31C after culturing on malt extract-glucose-yeast extract-peptone (MGYP) medium. The enzyme was purified by a combination of ultrafiltration and ion exchange chromatography on DEAE Sephadex and its molecular weight was estimated by gel filtration on Sephacryl S-300. The purified enzyme had a maximum yield of 50.84 % with a final purification of 70 folds. With a molecular weight of 214 KDa, it is composed of three subunits of 125, 60, and 25 KDa. The purified enzyme was successfully used for synthesis of silver nanoparticles in a way dependent upon NADPH using gelatin as a capping agent. The synthesized silver nanoparticles were characterized by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy. These stable nonaggregating nanoparticles were spherical in shape with an average size of 50 nm and a zeta potential of −34.3. Evaluation of the antimicrobial effects of synthesized nanoparticles by disk diffusion method showed strong growth inhibitory activity against all tested human pathogenic fungi and bacteria as evident from inhibition zones that ranged from 14 to 25 mm. Successful green synthesis of biologically active silver nanoparticles by a nitrate reductase from F. oxysporum in the present work not only reduces laborious downstream steps such as purification of nanoparticle from interfering cellular components, but also provides a constant source of safe biologically-active nanomaterials with potential application in agriculture and medicine.